S-layer-mediated association of Aeromonas salmonicida with murine macrophages
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چکیده
منابع مشابه
Aeromonas salmonicida resistance to complement-mediated killing.
The resistance of Aeromonas salmonicida to complement-mediated killing was investigated by using different strains and their isogenic mutants that had been previously characterized for their surface components. We found that the classical complement pathway is involved in serum killing of susceptible A. salmonicida strains, while the alternative complement pathway seems not to be involved. All ...
متن کاملTranscriptional analysis of the Aeromonas salmonicida S-layer protein gene vapA.
The vapA gene of Aeromonas salmonicida encodes the subunit of the surface protein array known as A-layer. Nucleotide sequence analysis of the 374 bp of DNA immediately upstream of vapA revealed two potential promoter sequences and other possible regulatory sequences. Sequencing and polymerase chain reaction analysis showed that the region was conserved in wild-type A. salmonicida. Primer extens...
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Insertion sequences (IS) are abundant in the bacterial fish pathogen Aeromonas salmonicida genome. IS are involved in rearrangement events that lead to the loss of virulence. In previous work, we studied a plasmid rearrangement that causes the deletion of the type three secretion system in A. salmonicida, resulting in a loss of virulence. We showed that the rearrangement is caused by the recomb...
متن کاملInteraction of Aeromonas spp. human isolates with murine macrophages.
This study aimed to evaluate phagocytic and bactericidal activity of macrophages originated from murine J774 macrophage cell line against 26 strains of Aeromonas caviae, Aeromonas veronii biotype sobria and Aeromonas hydrophila isolated from human fecal specimens. The analysis of phagocytic index (PI) indicated these strains were phagocytosed in low numbers, below 1.1 x l0(-3) for 65% strains o...
متن کاملSiderophore production by Aeromonas salmonicida.
Growth under conditions of iron-restriction and the production of siderophores was examined in 21 typical and 14 atypical strains of Aeromonas salmonicida. With the exception of one atypical strain, all strains grew and multiplied in the presence of the high-affinity iron chelators ethylenediamine di(o-hydroxyphenylacetic acid), alpha, alpha'-dipyridyl or transferrin. Chrome azurol S agar was u...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1992
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.60.10.4373-4382.1992